| 14706836 |
Talbot DA, Lambert AJ, Brand MD: Production of endogenous matrix superoxide from mitochondrial complex I leads to activation of uncoupling protein 3. J Biol Chem. 2007 Aug 17;282(33):24146-56. Epub 2007 Jun 20.
Superoxide generated using exogenous xanthine oxidase indirectly activates an uncoupling protein (UCP)-mediated proton conductance of the mitochondrial inner membrane. We investigated whether endogenous mitochondrial superoxide production could also activate proton conductance. When respiring on succinate, rat skeletal muscle mitochondria produced large amounts of matrix superoxide. Addition of GDP to inhibit UCP3 markedly inhibited proton conductance and increased superoxide production. Both superoxide production and the GDP-sensitive proton conductance were suppressed by rotenone plus an antioxidant. Thus, endogenous superoxide can activate the proton conductance of UCP3, which in turn limits mitochondrial superoxide production. These observations provide a departure point for studies under more physiological conditions. |
1(0,0,0,1) |