| 12515613 |
Wang L, Shao G, Zhang W, Guo X, Wang C, An J, Zhong G, Zhao H: [Influence of 4-aminopyridine on voltage-activated K (+) current and cell proliferation in small cell lung cancer]. Zhonghua Zhong Liu Za Zhi. 2002 May;24(3):230-3.
OBJECTIVE: To study the inhibition of voltage-activated K (+) conductance and cell proliferation by 4-aminopyridine (4-AP) in the human small-cell lung cancer (SCLC). METHODS: Inhibition of voltage-activated K (+) current by 4-AP through the whole-cell patch-clamp technique in SCLC cell line was studied. The influence on the cell-cycle by 4-AP was observed by flow cytometry to identify the in vitro inhibition by 4-AP to the cell proliferation of the SCLC cell line. RESULTS: Exposure of the tumor cells to 5 mmol/L 4-AP reduced the peak outward K (+) current (evoked by a depolarization to +80 mV) from 1.22 +/- 0.11 nA (n = 30) to 0.59 +/- 0.10 nA (n = 28). Flow cytometry results showed that cell population accumulated in the G (0)/G (1) phase and a significantly reduced proportion in the S phase and G (1)/G (2) phase cells after having been exposed to 4-AP for three days. Incubation of the SCLC cells with 0.1, 5, 10, 15, 20 mmol/L 4-AP resulted in a concentration-and time-dependent reduction in the number of viable cells as compared with the control. CONCLUSION: The voltage-activated K (+) channels expressed by SCLC play an important role in SCLC cell proliferation. The proliferation of the SCLC cells is inhibited by K (+) channel antagonists. |
36(0,1,1,6) |